Session Index

Biophotonics and Biomedical Imaging

Biophotonics and Biomedical Imaging III
Friday, Dec. 3, 2021  10:45-12:00
Presider: Biomedical Imaging III: Prof. Yuan Luo & Dr. Bi-Chang Chen
Room: 303b
10:45 - 11:00
Manuscript ID.  0538
Paper No.  2021-FRI-S0603-O001
Tien-Yu Wu Distortion correction based on division model for sCMOS cameras in CB-XLCT
Chia-Lun Tsai;Tien-Ai Ji;Jyh-Cheng Chen;Tse-Ying Liu;Tien-Yu Wu;Snow H. Tseng

In our cone-beam X-ray luminescence computed tomography (CB-XLCT) system, luminescent optical imaging was affected by lens distortion, which caused great difficulty in the direct interpretation of the image. When the magnification of the lens decreases with axial distance causing, radial distortion occurs, causing each image point to move radially towards the center of the image. Thus, it is essential to use Geometric calibration to correct the image. Therefore, Fitzgibbon’s division model will be employed to solve the initial values of distortion for presentation of real data.

11:00 - 11:15
Manuscript ID.  0252
Paper No.  2021-FRI-S0603-O002
Zi-Ping Chen UV-visible-NIR self-phase-modulation-enabled femtosecond fiber source for biomedical single/two-photon fluorescence microscopy
Zi-Ping Chen;Lu-Ting Chou;Yu-Cheng Liu;Shih-Hsuan Chia

We optimized the spectral coverage of self-phase-modulation-enabled femtosecond fiber sources by careful investigations into the influence of input pulse width, fiber length, and fiber damage, and we have demonstrated a widely tunable source ranging from 740-1250 nm for two-photon microscopy applications. In addition, tens of milliwatt tunable near-UV/visible spectrum is easily obtained by a frequency-doubled conversion, and the gap between the fundamental and frequency-doubled spectra can be filled with the nonlinear wave breaking around 650 nm. A multi-modality microscopy incorporating two-photon microscopy and confocal fluorescence microscopy was also demonstrated to prove the versatility of our development for biomedical imaging.

11:15 - 11:30
Manuscript ID.  0695
Paper No.  2021-FRI-S0603-O003
Yu-Chun Chen Three-dimensional fluorescence imaging of Caenorhabditis elegans by Bessel beam light sheet microscopy
Yu-Chun Chen;Sunil Vyas;Kuang-Yuh Huang;Yuan Luo

To image three-dimensional (3D) living organisms, optical sectioning and high-speed image acquisition are required. Using Bessel light sheet microscopy, we experimentally demonstrate 3D real-time fluorescence imaging of Caenorhabditis elegans (C. elegans). High resolution images obtained with our system can resolve fine structural details to perform developmental biological studies.

11:30 - 11:45
Manuscript ID.  0690
Paper No.  2021-FRI-S0603-O004
Yen-Chih Yu Design and Development of FPGA controlled Quantitative Differential Phase Contrast Microscope
Yen-Chih Yu;Sunil Vyas;J. Andrew Yeh;Yuan Luo

Isotropic quantitative differential phase contrast microscopy (iDPC) requires an optical system, electronic hardware to control illumination, and computational post-processing to reconstruct quantitative phase images. To make the system compact and portable, an efficient strategy is required. Here, we propose FPGA based iDPC microscopy which is designed and developed for biomedical imaging. The FPGA system is used to perform three basic operations: illumination control, image acquisition, and image reconstruction. Our system can provide high speed phase contrast images suitable for label-free live cell imaging.

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11:45 - 12:00
Manuscript ID.  0532
Paper No.  2021-FRI-S0603-O005
Tien-Ai Ji Comparison of algebraic ART, SART and SIRT image reconstruction methods in CB-XLCT
Tien-Ai Ji;Chia-Lun Tsai;Tien-Yu Wu;Snow H. Tseng;Jyh-Cheng Chen;Tse-Ying Liu

The measurement of soft tissue in biological body is a known problem. To solve this problem, we developed a Cone-beam X-ray luminescence computed tomography (CB-XLCT) system, a dual modality technique, which can measure the hard and soft issue at the same time. To reconstruct the optical data of luminescent tomography, we employed Filtered Back Projection (FBP) method, and we made comparison between the constructions of soft tissue by three iterative reconstruction techniques: Algebraic Reconstruction Technique (ART), Simultaneous Algebraic Reconstruction Technique (SART) and Simultaneous Iterative Reconstruction Technique (SIRT).